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. 2023 Jun 15;7(2):026112. doi: 10.1063/5.0153214

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© 2023 Author(s).

All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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FIG. 5. — Cell tracking results for zebrafish beating heart. (a) Raw and segmented images of Tg(cmlc2:nucGFP) zebrafish heart at 7 dpf were presented as a MIP image. The total number of tracked cells is 580. (b) Cell tracking algorithm enabled us to register cells among various cardiac phases. Manually corrected cells in the first volume were denoted as red circles, while the cells registered by 3DeeCellTracker in target next volume were denoted as blue crosses. (c) Assessment of the tracking accuracy in 64 cells over time. The results indicate false positive (FP) labels where non-cell regions were labeled as cells and false negative (FN) labels where specific cells were not detected. Individual cells were assigned pseudo-colors to differentiate them. (d) and (e) Further displacement analysis allowed for the tracking of representative cells in the ventricle, atrium, and AVC in the 3D space at 3 and 7 dpf, respectively.