Figure 5.

Copper promotes ferroptosis in nude mice bearing a PANC-1 xenograft. (A-C) Subcutaneous growth of PANC-1 xenografts in nude mice treated with IKE (30 mg/kg, i.p., once every other day), copper gluconate (Cu, 0.15 mg/kg, i.g., once every day), or liproxstatin-1 (Lip-1, 10 mg/kg, i.p., once every day). (A) Tumor growth curves of PANC-1 xenografts in nude mice treated with the indicated agents. *P < 0.05. Representative images of tumors (B) and tumor weight (C) were shown. Data are presented as mean ± SD, n = 8 mice per group; *P < 0.05; ns, no significance. (D) Immunohistochemical staining and relative staining intensity of the indicated proteins in the tumor sections. *P < 0.05; ns, no significance.