Figure 6.

Copper chelator inhibits ferroptosis-associated acute pancreatitis in mice. (A) Representative images of pancreatic histology in L-arginine-induced pancreatitis in mice with or without TM treatment. Scale bar: 200 µm. (B-D) Serum AMY (B), pancreatic MPO activity (C), or serum HMGB1 (D) in control or L-arginine-induced-acute pancreatitis mice treated with or without TM (0.7 mg per mouse, i.g., once every day). Data are presented as mean ± SD; n = 6 mice/group; *P < 0.05 versus control group. (E) Representative immunohistochemical (IHC) staining of pancreatic tissues from control or acute pancreatitis mice treated with or without TM. In addition, iron content (blue) in tissues was detected using an iron staining kit. The relative expression of GPX4, 4-HNE, TFRC or iron was quantified from 3 mice. *P < 0.05.