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. 2023 Jan 1;19(7):1916–1933. doi: 10.1080/15548627.2022.2162798

Figure 7.

Figure 7.

Tyrosine at position 313 of NP is the key residue for the interaction of MAVS with NP. (A) HEK293T cells were transfected with PR8-NP or PR8-NPY313F before co-IP with anti-Flag antibody. (B) A549 cells were infected with rPR8-NPY313F virus for the indicated times before co-IP with anti-MAVS antibody. (C) U2OS cells were infected with rPR8 or rPR8-NPY313F virus, respectively. Twenty hours later, the cells were fixed and stained with anti-NP and anti-TOMM20 before confocal microscopy. Scale bar: 20 μm. The right panel shows the quantification of Pearson’s colocalization coefficient between NP and TOMM20. (D) U2OS cells were infected with rPR8 or rPR8-NPY313F virus, respectively. Twenty hours later, the cells were fixed and stained with anti-NP and anti-MAVS before confocal microscopy. Scale bar: 20 μm. The right panel shows the quantification of Pearson’s colocalization coefficient between NP and MAVS. (E) U2OS cells were infected with rPR8 or rPR8-NPY313F virus, respectively. Twenty hours later, the cells were fixed and stained with anti-NP and anti-TOLLIP before confocal microscopy. Scale bar: 20 μm. The right panel shows the quantification of Pearson’s colocalization coefficient between NP and TOLLIP. Results are representative of three independent experiments.