Figure 3.

TRIM21 and G3BP1 interact with several autophagy machinery proteins upon arsenite treatment. (A-E) HEK293FT cells were transfected with EGFP-ULK1 (A), EGFP-BECN1 (B), EGFP-MAP1LC3B (C), EGFP-SQSTM1 (D) or MYC-CALCOCO2 (E), along with the empty vector or FLAG-TRIM21, and the cells were treated without or with arsenite. FLAG affinity isolation was performed with anti-FLAG beads, and the samples were analyzed by immunoblotting with the indicated antibodies. (F) HEK293FT cells were treated without or with arsenite for 1 h. Anti-G3PB1 immunoprecipitation was performed, and the samples were analyzed by immunoblotting with the indicated antibodies. Blots are representative of three biological replicates. Relative fold changes of the immunoprecipitated proteins are presented as means of three replicate experiments ± SEM. Significance was determined using Student’s t-test (A-F); *p < 0.05, **p < 0.01, ***p < 0.001.