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. 2023 Feb 10;19(7):2078–2093. doi: 10.1080/15548627.2023.2170962

Figure 9.

Figure 9.

SNAP29 O-GlcNAcylation promotes ROS-mediated cell death. (A) Representative cytofluorimetric plots of DHE fluorescence in HT1080 treated with SM15 for different incubation times or treated with the ROS producer H2O2 (5 mM) for 30 min. (B) Quantification of DHE-positive HT1080 cells treated as in (A). *P < 0.05 (6 h vs. 24 h). (C) Quantification of DHE-positive HeLa cells treated with SM15 for 24 h with or without 5 mM alloxan. ***P < 0.001 (- alloxan vs. + alloxan). (D) Representative cytofluorimetric plots of ANXA5 fluorescence in SNAP29-WT or SNAP29-QM expressing HeLa cells. Cells were treated with SM15 for the last 24 h of the expression time, NAC was added 30 min before SM15. Boxed areas represent ANXA5-positive cells. (E) Quantification of ANXA5-positive cells treated as in (D). **P < 0.01 (SNAP29-WT vs. SNAP29-QM).