FIGURE 5.
CVM-1125 binds strongly to TRAP1 (A) To identify CVM-1125 binding target, the NPOT assay was performed on CVM-1125 treated MCF7 and COLO205 cells, as well as the colorectal and melanoma tumor homogenates. The experiments were performed three times independently. Arrows point at the isolated drug specific macromolecular assemblies. (B) The binding between TRAP1 and CVM-1125 was confirmed using SPR. There is a dose-dependent interaction between CVM-1125 and the immobilized TRAP1 with K D of 6.7 × 10−8 M. However, no specific interaction of Hsp90 to CVM-1125 was observed. (C) In silico interaction analysis of CVM-1125 with TRAP1 at the N-terminal domain (NTD).