Figure 7.

Comparison of FRET systems between pyrene–perylene and FITC–TAMRA. (A) Emission spectra of the hybrid between Pe-P₅/Py-P₃ and Tgt 32mer with 347-nm excitation, and (B) emission spectra of the hybrid between FITC-P₃/TAMRA-P₅ and a target 36mer with 499-nm (for +DMF) and 494-nm excitations (for –DMF) in 10 nM phosphate buffer, pH 7.0 containing 0.2 M NaCl in the presence and absence of 20% (v/v) DMF at 25°C. The concentration of each dye-labeled probe and target was 100 nM. In (B), the 36mer which had four excess thymine oligonucleotides in the center of Tgt 32mer was used since this 36mer afforded the highest energy transfer efficiency.