Figure 5.

Characterization of PET films upon degradation by IsPETaseTM

(A and B) Area variation (blue lines), G″ and G′ measurement (orange line), and compression speed (gray line) over time during degradation of PET by IsPETaseTM at a final concentration of 0.25 (A) and 0.5 μg/mL (B).

(C) In situ BAM images during stage I degradation. Time indicates the incubation time with the enzyme, and the arrowheads show selected locations on the film being degraded through time. Scale bar, 50 µm

(D) AFM of films transferred at the end of degradation (stage IV) at 40°C (left) and 50°C (right). From left to right: 1 × 5 and 1 × 1 μm scan. The dark regions represent holes in the film due to transfer at high temperatures.