Figure 5.

Select protein marker expression, conditional on cell type (stratified by median expression), predictive of nodal metastasis: CD34 expression stratified by programmed death ligand 1 (PD-L1)–expressing cells inside the tumor (Intra; A), CD44 stratified by CD3 at the interface (Inter; B), and CD127 stratified by CD66b at the interface (C). Marker expression plotted in beeswarm plots was filtered on the basis of the detection of outliers using a modified Tukey outlier test—after this initial filtering, only points between the 10% and 90% quantiles for each stratum were included. Intra and Inter are described in Data Acquisition and Preprocessing.