Figure 2.

Effects of acute exposure to MC-LR on follicle ovulation. (A) The schematic of intraperitoneal treatment of $1\times$ PBS ($N=9$) or $10\mathrm{\mu }\mathrm{g}/\mathrm{kg}$ MC-LR ($N=10$) in a mouse superovulation model. (B) Numbers and (C) representative images of ovulated oocytes from mice treated with $1\times$ PBS or MC-LR. (D) Percentages of ovulated MII oocytes in mice treated with $1\times$ PBS or MC-LR. (E) Representative ovarian histological images after ovulation induction in $1\times$ PBS- or MC-LR–treated mice. Blue dash squares indicate newly formed corpora lutea (CL) or un-ovulated late-staged antral follicles in $1\times$ PBS- or MC-LR–treated mouse ovaries, respectively. (F) Total numbers of un-ovulated late-staged antral follicles per mouse in $1\times$ PBS- or MC-LR–treatment groups. $N=4$ mice or 8 ovaries were randomly selected in each group for the histological staining and counting of un-ovulated follicles. Data were analyzed with Student’s $t$-test (B,D,F). Bidirectional error bars represent $\text{mean}\pm \text{standard deviation}$; *$p<0.05$ and **$p<0.01$. Data in (B,D,F) are also presented in Tables S6–S8, respectively. Note: hCG, human chorionic gonadotropin; IP, intraperitoneal; MC-LR, microcystin-LR; MII, metaphase II; PBS, phosphate-buffered saline; PMSG, pregnant mare serum gonadotropin.