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. 2023 Jun 21;131(6):067010. doi: 10.1289/EHP12034

Figure 4.

Figure 4A is a stained tissue displaying two rows, namely, Control and MC-LR with a scale bar of 50 micrometers. Figure 4B is a dot plot titled Follicle development, plotting Follicle diameter (micrometers), ranging from 300 to 550 in increments of 50 (y-axis) across Control and MC-LR (x-axis). Figure 4C is a stained tissue displaying two columns, namely, Control and MC-LR with a scale bar of 100 micrometers. Figure 4D is a set of eight dot plots. On the top, the four graphs are titled Inha, Inhba, Inhbb, and Comp, plotting Relative messenger ribonucleic acid expression, ranging from 0 to 150 in increments of 50; 0 to 150 in increments of 50; 0 to 10 in increments of 2; and 0 to 4 in unit increments (y-axis) across Control and MC-LR. At the bottom, the graphs are titled Cyp19a1, Fshr, Lhcgr, and Pappa, plotting Relative messenger ribonucleic acid expression, ranging from 0 to 30 in increments of 10; 0 to 6 in increments of 2; 0 to 15 in increments of 5; and 0 to 1.5 in increments of 0.5 (y-axis) across Control and MC-LR. Figure 4E is a set of one stained tissue and one bar graph. The stained tissue is titled L H C G R and displays two columns, namely, Control and MC-LR with a scale bar of 100 micrometers. The bar graph, plotting Relative expression, ranging from 0 to 50 in increments of 10 (y-axis) across Control and MC-LR. Figure 4F is a set of one stained tissue and one bar graph. The stained tissue is titled PA P P A and displays two columns, namely, Control and MC-LR with a scale bar of 100 micrometers. The bar graph, plotting Relative expression, ranging from 0 to 40 in increments of 10 (y-axis) across Control and MC-LR.

MC-LR accumulation in the ovary and examination of follicle maturation in response to MC-LR exposure. (A) Ovarian accumulation of MC-LR examined by immunohistochemistry (IHC). (B) Diameters of late-staged antral follicles isolated from mice treated with 1× PBS (N=91 follicles from 6 ovaries) and MC-LR (N=95 follicles from 8 ovaries). (C) Representative histological images of late-staged antral follicles in ovaries from 1× PBS- or MC-LR–treated mice. (D) Expression of follicle maturation-related genes in isolated large antral follicles from 1× PBS- or MC-LR–treated mice. N=24 and 25 follicles were isolated from 5 mice treated with 1× PBS and MC-LR, respectively. Expression and quantification of (E) LHCGR and (F) and PAPPA in late-staged antral follicles in 1× PBS- or MC-LR–treated ovaries examined by IHC. Data were analyzed with Student’s t-test (B,D,E,F). Bidirectional error bars represent mean±standard deviation; *p<0.05 and **p<0.01. Data in (B,D–F) are also presented in Tables S11, S13–S15, respectively. Note: MC-LR, microcystin-LR; PBS, phosphate-buffered saline.