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. 2023 Jun 8;19(6):e1011185. doi: 10.1371/journal.ppat.1011185

Table 1. Summary of UL138 Interactorsa.

UL138 Interactor Cellular Functions Peptideb Scansc Coveraged
WDR48 (UAF1) Scaffold required for USP1, USP12, and USP46 activity
25 74 39.7
WDR20 Enhances USP12 and USP46 activity; regulates nuclear trafficking of USP12
10 26 20.7
USP12 Sustains pSTAT1 through inhibition of CBP acetyltransferase activity required for dephosphorylation of pSTAT1 by PTPN2
9 26 19.7
EGFR Activates PI3K/AKT, MEK/ERK, JAK/STAT, and PLCγ signaling 8 9 8.9
JAK1 Phosphorylates STAT1
3 3 2.8
UL138 Human Cytomegalovirus early protein that promotes viral latency
8 128 44.9

aCandidate interactions determined by IP/MS from infected fibroblasts (TB40/E-UL138FLAG) (MOI = 3) at 48 hpi. UL138FLAG was immunoprecipitated with a Flag antibody and following tryptic digest, peptides were identified by IP-MS/MS. Interacting candidates were subtracted from a control Flag pull-down from infected fibroblasts without a Flag tagged UL138. 128 candidates were identified and top interactions were identified by STRING and NCBI analysis. Top-ranking candidates involved in STAT1 signaling are listed based on the peptide count, scan count, and percent coverage.

bNumber of unique peptides

cNumber of scans identifying a unique peptide

dPercent of the protein covered by the identified peptides