Table 1. Summary of UL138 Interactorsa.
UL138 Interactor | Cellular Functions | Peptideb | Scansc | Coveraged |
---|---|---|---|---|
WDR48 (UAF1) | Scaffold required for USP1, USP12, and USP46 activity |
25 | 74 | 39.7 |
WDR20 | Enhances USP12 and USP46 activity; regulates nuclear trafficking of USP12 |
10 | 26 | 20.7 |
USP12 | Sustains pSTAT1 through inhibition of CBP acetyltransferase activity required for dephosphorylation of pSTAT1 by PTPN2 |
9 | 26 | 19.7 |
EGFR | Activates PI3K/AKT, MEK/ERK, JAK/STAT, and PLCγ signaling | 8 | 9 | 8.9 |
JAK1 | Phosphorylates STAT1 |
3 | 3 | 2.8 |
UL138 | Human Cytomegalovirus early protein that promotes viral latency |
8 | 128 | 44.9 |
aCandidate interactions determined by IP/MS from infected fibroblasts (TB40/E-UL138FLAG) (MOI = 3) at 48 hpi. UL138FLAG was immunoprecipitated with a Flag antibody and following tryptic digest, peptides were identified by IP-MS/MS. Interacting candidates were subtracted from a control Flag pull-down from infected fibroblasts without a Flag tagged UL138. 128 candidates were identified and top interactions were identified by STRING and NCBI analysis. Top-ranking candidates involved in STAT1 signaling are listed based on the peptide count, scan count, and percent coverage.
bNumber of unique peptides
cNumber of scans identifying a unique peptide
dPercent of the protein covered by the identified peptides