Skip to main content
. 2023 Jun 7;11:1045759. doi: 10.3389/fcell.2023.1045759

FIGURE 5.

FIGURE 5

The ligand-dependent S1R proximatome. (A) A volcano plot comparing the proximatome of S1R-Apex under untreated conditions versus treatment with 25uM Haloperidol for 24 h. A line demarcating 2-fold enrichment and a p-value of 0.05 is shown. The grey shaded box indicates proteins that show at least 2-fold greater binding to S1R under Haloperidol treatment conditions with a p-value of at least 0.05. A list of these proteins is shown below the volcano plot. (B) A volcano plot comparing the proximatome of S1R-Apex under untreated conditions versus treatment with 20uM (+)-PTZ for 24 h. The layout is similar to panel (A). The proteins in blue were shown to have an increased proximal association with S1R under both Haloperidol and (+)-PTZ treatment conditions. (C–E) Biotinylated proteins were purified from HeLa cells stably expressing either GFP-Apex or S1R-Apex that were untreated or treated with either Haloperidol or (+)-PTZ. The proteins were purified using streptavidin conjugated beads. The bound proteins were eluted and analyzed by Western blotting using antibodies against PCSK9 (C), LDLR (D) or Thrombospondin1 (E). Haloperidol treatment increased the proximal association between S1R and PCSK9, whereas (+)-PTZ treatment increases the proximal association between S1R LDLR and Thrombospondin1.