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. 2023 Jan 13;30(6):503–519. doi: 10.1038/s41434-022-00380-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Vectors have the Kozak consensus sequence (GCCACCATGG) surrounding the start codon of eGFP, if indicated by the grey vertical line. A polyadenylation signal is located downstream of the transgene in all investigated vectors. The packaging cassettes are flanked by inverted terminal repeats (ITR). The investigated viral vectors did not have chimeric introns or a woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) for promoter comparison purposes and to make incorporation of potential large transgenes possible. bp base pair, pA polyadenylation signal.