Fig. 2. The effect of the Kozak consensus sequence on eGFP protein expression in the retina.
A eGFP (27 kDa) and loading control β-actin (45 kDa) were assessed in retinal lysates 4 weeks after intravitreal injection of 5 × 109 genome copies per eye of AAV2-CBA-eGFP excluding the Kozak sequence (n = 4) or AAV2-CBA-eGFP including the Kozak sequences (n = 4). Eyes that were not injected were processed identically as naïve controls (n = 2). B Quantification of the mean eGFP intensity per transduced retina is shown, corrected for total protein loaded per sample (df = 6, t = 2.93, p < 0.05, Student’s t test for No Kozak vs. Kozak). The grey bars depict the mean ± SEM. Each individual data point represents one retina. Images were taken from the same Western blot; different exposure times were needed to visualise β-actin and eGFP proteins, respectively. *p < 0.05.