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. 2023 Jan 13;30(6):503–519. doi: 10.1038/s41434-022-00380-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Schematic representation of a ventral view on the mouse brain, in which the white square illustrates the location of the optic chiasm and related structures. B Microscopic overview of the optic chiasm from a mouse that had bilateral AAV2-SYN-eGFP intravitreal injections. This image is modified from the panel containing AAV2-SYN-eGFP in Fig. 3C. C eGFP-positive fibres were found at the optic nerve, optic chiasm and optic tract in all investigated experimental groups. All images were taken using a tile-scanning epifluorescence microscope with exposure settings selected to maximise axon visualisation for each promoter. Horizontal sections were stained for DAPI, eGFP is shown throughout without amplification using a GFP antibody. Schematic was created with BioRender.com.