Fig. 3. Dynamics of myeloid cell changes during UC.

a DSS treatment schedule. Mice were treated with 1.5% or 3% DSS in drinking water, followed by a four-week chase period with normal drinking water. Colon tissue was isolated and analyzed at indicated time points. Weight loss and the presence of occult blood in the feces were used as indicators for the successful establishment of DSS-induced colitis model.Weight data (ctrl, n = 5; 3% DSS, n = 5 from one experiment) were shown as mean +/− SD and performed with two-way ANOVA test (***P < 0.001). Exact P values were provided in the Source Data file. b Gating strategy for different myeloid cells in normal colon tissue at day 0. CD11b⁺ F4/80^hi Fraction I cells were separated into MHC II⁻ and MHC II⁺ subsets of resident macrophages. CD11b⁺ F4/80^int Fraction II cells were separated into P1 (Ly6c⁺ MHC II⁻ monocytes), P2 (Ly6c⁺ MHC II⁺ inflammatory macrophage), P3 (Ly6c⁻ MHC II⁺ infiltrating macrophage), and P4 (Ly6c⁻ MHC II⁻ eosinophil). CD11b⁺ F4/80⁻ Fraction I cells were neutrophils. c–e Representative FACS plots, subset percentages, and absolute numbers for the different myeloid cells shown in Fig. 5B during different time points of the DSS model. Data (1.5% DSS, n = 5; 3% DSS, n = 5 from one experiment) were shown as mean +/− SD. f Representative FACS plots and statistic results of infiltrating macrophages in WT (n = 5) and CCR2 knockout mice (n = 5 from one experiment) after DSS-induced colitis. Data were shown as mean +/− SD and performed with two-tailed T test (ns P > 0.05, *P < 0.05, **P < 0.01). Exact P values were provided in the Source Data file.