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. 2023 Jun 21;14(6):368. doi: 10.1038/s41419-023-05891-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Western blotting of WCLs of AN3 CA cells with FBXO7 knockout and transfected with pCDH-CD513B-FBXO7-WT/D72G plasmids, or parental. All quantitation were normalized to the protein level of endogenous parental GAPDH (Left). Cell proliferation assay of AN3 CA cells with FBXO7 knockout and transfected with pCDH-CD513B-FBXO7-WT/D72G plasmids, or parental (Right). Data are shown as means ± SD (n = 5). *p < 0.05. B Western blotting of WCLs of HEC-1-A cells with FBXO7 knockout and transfected with pCDH-CD513B-FBXO7-WT/D72G plasmids, or parental (Left). All quantitation were normalized to the protein level of endogenous parental GAPDH. Cell proliferation assay of AN3 CA cells with FBXO7 knockout and transfected with FBXO7-WT/D72G, or parental (Right). Data are shown as means ± SD (n = 5). *p < 0.05. C Cell colony formation assay of AN3 CA cells with FBXO7 knockout and transfected with pCDH-CD513B-FBXO7-WT/D72G plasmids, or parental (Left). Statistics of cell colony formation assay (Right). Data are shown as means ± SD (n = 3). *p < 0.05. D Cell colony formation assay of HEC-1-A cells with FBXO7 knockout and transfected with pCDH-CD513B-FBXO7-WT/D72G plasmids, or parental (Left). Statistics of cell colony formation assay (Right). Data are shown as means ± SD (n = 3). *p < 0.05. E Cell migration assay of AN3 CA cells with FBXO7 knockout and transfected with pCDH-CD513B-FBXO7-WT/D72G plasmids, or parental (Left). Statistics of cell migration assay (Right). Data are shown as means ± SD (n = 3). *p < 0.05. F Cell migration assay of HEC-1-A cells with FBXO7 knockout and transfected with pCDH-CD513B-FBXO7-WT/D72G plasmids, or parental (Left). Statistics of cell migration assay (Right). Data are shown as means ± SD (n = 3). *p < 0.05. Experiments in (A, B) were repeated three times.