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. 2023 Jun 21;9:48. doi: 10.1038/s41526-023-00286-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Addition of hydrocortisone (HC) or a control vehicle (PBS) to the medium was done 48 h before exposure to altered gravity levels and was followed by exposure to simulated microgravity (smg) by means of the Random Positioning Machine or hypergravity at 15 × g or 20 × g by means of the Large Diameter Centrifuge (LDC) located at ESA-ESTEC. Two additional groups were exposed to simulated microgravity intermitted with a temporary hypergravity exposure at either 15 × g T or 20 × g T after which they were placed back into simulated microgravity. Finally, 1 × g controls were placed in the center of the LDC. This figure was created with BioRender.com.