Table 1.
List of Cannabis sativa studies reporting micropropagation from pre-existing meristems.
| Type of explant | Genotype (Type) | Number of shoots obtained | References |
|---|---|---|---|
| Nodal segments | MX-1 (drug) | 13.8 shoots/explant | Lata et al., 2009a |
| Shoot tips | Changtu (fiber) | 3.22 shoots/shoot tip | Wang et al., 2009 |
| Nodal segments | Mexican variety (drug, THC rich) | 13.44 shoots/explant | Lata et al., 2016 |
| Immature and mature floral explants | 1KG2TF (drug, THC rich) | 4a shoots/immature inflorescence | Piunno et al., 2019 |
| S1525 (drug, THC rich) | < 1a shoot/immature inflorescence | ||
| Isolated meristem | USO-31 (fiber, < 3% CBD) | 4.4 shoots/explant | Smýkalová et al., 2019 |
| Shoot apex | 3.2 shoots/explant | ||
| Cotyledonary node | 2.4 shoots/explant | ||
| Nodal segments | High CBD variety | 3.63 shoots/explant | Ioannidis et al., 2020 |
| High CBG variety | 3.38 shoots/explant | ||
| Nodal segments | MX-CBD-11 (drug, CBD rich) | 3.00 shoots/explant | Mestinšek-Mubi et al., 2020 |
| MX-CBD-707 (drug, CBD rich) | 2.43 shoots/explant | ||
| Shoot tips of epicotyl | Diana | 3.4 shoots/explant | Dreger and Szalata, 2021 |
| Fedora 17 | 3.2 shoots/explant | ||
| Finola (all hemp) | 3.7 shoots/explant | ||
| 2 cm stem tips | Wife (hemp) | 8.4 micro-cuttings/4 explants | Lubell-Brand et al., 2021 |
| Meristems | Pure CBD | 1.9 shoots/explant | Holmes et al., 2021 |
| Nodal segments | Cheesequake | 0.5 shoots/explant | |
| Moby Dick | 1.9 shoots/explant | ||
| Space Queen | 0.2 shoots/explant | ||
| Pennywise (all drug type) | 2.0 shoots/explant | ||
| BLD | 0.4 shoots/explant | ||
| SWD | 0.4 shoots/explant | ||
| Moby Dick (all drug type) | 0.3 shoots/explant | ||
| Single floret | U82 (drug, THC rich) | 18.2 shoots/explant | Monthony et al., 2021a |
| Pair of florets | 14.7 shoots/explant | ||
| Shoot tip | US Nursery Cherry I (drug) | 5.75 shoots/explant, over 4 cycles | Murphy and Adelberg, 2021 |
| Two-node explants | BA-21 (drug, code name) | 2.23 shoots/explant | Page et al., 2021 |
| BA-41 (drug, code name) | |||
| BA-49 (drug, code name) | |||
| BA-61 (drug, code name) | |||
| BA-71 (drug, code name) | |||
| Tip cuttings | Epsilon 68 (fiber, CBD rich) | 2.5 shoots/explant | Wróbel et al., 2022 |
| Nodal cuttings | 1.3 shoot/explant | ||
| Secondary tip and nodal cuttings | 3 shoots/explant | ||
| Microshoots | Abacus (hemp) | 2.6 shoots/explant | Borbas et al., 2023 |
| Wife (hemp) | 3.7 shoots/explant | ||
| Stem segments with two nodes | Honey Banana (THC-rich) | 1.5 shoot/explant | Hesami et al., 2023 |
| 1-cm single-node stem segment | TJ’s CBD (CBD-rich) | >20a shoots/explant (TDZ, low quality) | Stephen et al., 2023 |
| 8a shoots/explant (BA, high quality) | |||
| Shoot segments with one node | Safari Punch (THC-dominant) | 5.33 shoots/explant | Zarei et al., 2023 |
| Peanut Sundae (THC-dominant) | 2a shoots/explant | ||
| Master Hemp (CBD-dominant) | 5.4 shoots/explant | ||
| Orange Mimosa Purple (THC rich) | 2.4a shoots/explant | ||
| Golden Papaya (THC-dominant) | 3.33 shoots/explant |
Only genotypes from which shoots were obtained are included. a number estimated from figure.