Table 2.
List of Cannabis sativa studies reporting regeneration experiments without transformation.
| Type of explant | Genotype | Type | Callus stage | Best outcome | References | |
|---|---|---|---|---|---|---|
| Responding explants (%) | No. of shoots per explant | |||||
| Axillary bud Petiole Internode |
Fibrimon-24, Silesia, Novosadska, Fedrina-74 Juso-15 |
Hemp Hemp Hemp |
Yes Yes Yes |
2.3 2.5 1.5 |
Slusarkiewicz-Jarzina et al., 2005 | |
| Stem Cotyledon |
Bialobrzeskie, Beniko, Silesia Bialobrzeskie, Beniko, Silesia |
Hemp Hemp |
Yes Yes |
14 6 |
Wielgus et al., 2008 | |
| Young leaf | MX | Drug | Yes | 96.6 | 12.3 | Lata et al., 2010 |
| Epicotyl Cotyledon |
Iranian Cannabis | Unknown | Yes Yes |
2 1 |
Movahedi et al., 2015 | |
| Cotyledon | Kunming, Neimeng 700, YM535, Anhui727, DaliA1, Heilongjiang698, Heilongjiang449 BM2 |
Hemp Hemp Seed |
Yes | 54.8 | 3.0 | Chaohua et al., 2016 |
| Hypocotyl Cotyledons Leaf |
Ferimon, Felina32, Fedora17, USO31, Finola Ferimon, Felina32, Fedora17, USO31, Finola Ferimon, Felina32, Fedora17, USO31, Finola |
Hemp Hemp Hemp |
No No No |
71.15 9.29 average 0.42 |
1.72 1.42 - |
Galán-Ávila et al., 2020 |
| Hypocotyl Cotyledons |
Ferimon, Felina32, Fedora17, USO31, Finola, Futura75 Ferimon, Felina32, Fedora17, USO31, Finola, Futura75 |
Hemp Hemp |
No No |
76.5 26.3 |
1.6 2.0 |
Galán-Ávila et al., 2021b |
| Leaf segments | UP305 | Drug | Yes | 18 | Hesami and Jones, 2021 | |
| Stem nodes | Samsun Vezirköprü population | Drug | Yes | 75 | 4.593 | Önol and Yildirim, 2021 |
| True leaves Cotyledon Hypocotyl Embryo hypocotyls of immature grains |
YUNMA7 One hundred Cannabis varieties |
Hemp Hemp |
Yes Yes Yes Yes |
2.27 1.78 1.41 7 |
Zhang et al., 2021 | |
| Stem | H-CBD variety H-CBG variety |
Drug Drug |
Yes Yes |
62 | Ioannidis et al., 2022b | |