Table 3.
List of Cannabis sativa studies reporting transient transformation.
| Type of explant | Method | Genotype | Gene of interest | References |
|---|---|---|---|---|
| Part of leaf Small plants |
Agroinfiltration | Unknown | EGL3 | Roscow JR., 2017 |
| Cotyledons Leaves |
Agroinfiltration | Finola (hemp) | PDS (VIGS) Chl1 (VIGS) |
Schachtsiek et al., 2019 |
| Various tissue incl. leaves, male and female flowers | Agroinfiltration | CRS-1; CFX-2; Fedora 17; Felina 32; Ferimon; Futura 75; Santhica 27; Uso31 All hemp |
pEarlyGate101-uidA (GUS) PDS (RNAi) |
Deguchi et al., 2020 |
| Intact seedlings | Agroinfiltration | Nightingale Green Crack CBD Holy Grail x CD-1 All drug type |
pCAMBIA1301 with uidA (GUS) | Sorokin et al., 2020 |
| Leaves | Vacuum infiltration with gold nanoparticles | Tygra (THC low) | GmMYB29A2 GmNAC42-1 |
Ahmed et al., 2021 |
| Leaves | Protoplasts/PEG | Cherry x Otto II: Sweetened CDB high; THC low |
pBeaconGFP_GR-GUS pEVTV_DR5 pBeaconRFP_GUS |
Beard et al., 2021 |
| Leaf segments | Agroinfiltration | Cannbio-2 (THC : CBD ratio 1:1.8) |
pRNAi-GG-THCAS pRNAi-GG-CBDAS pRNAi-GG-CBCAS pRNAi-GG-CBDAS-UNIVERSAL |
Matchett-Oates et al., 2021b |
| Leaves | Protoplasts/PEG | THC high genotype | GFP | Matchett-Oates et al., 2021a |
| Leaves | Protoplasts/PEG | Abacus (CBD rich) | CsCBAS : GFP CsCBDAS : GFP CsTHCAS : GFP |
Kim et al., 2022 |
| Cotyledons | Protoplasts/PEG | Blueberry Divine (THC : CBD ratio: 20:1) CAN 40 (6:5) CAN 21 (3:100) Charlotte’s Web (85:1) Mantanuska Divine (3:2) Divine (43:100) Lemon Divine (12:5) CAN 20 (89:1) Yunma No. 1 (hemp) |
CsMYC2 | Zhu et al., 2022 |