FIGURE 3.

Nicotine-containing e-cigarette vapour (ECV) inhalation increased endothelial permeability in isolated perfused and ventilated mouse lungs. a) Effect of nicotine-free e-cigarette vapour (NF ECV) or ECV on hypoxic pulmonary vasoconstriction, determined as the maximum increase of pulmonary arterial pressure (ΔPAP) during hypoxic ventilation. b) Effect of NF ECV or ECV on the capillary filtration coefficient (K_fc), which was measured gravimetrically and calculated from the slope of lung weight gain induced by an increase of the pulmonary venous pressure from 2 mmHg to 10 mmHg. Data are provided as percentage change of ΔPAP and K_fc compared to the reference hypoxic manoeuvre or pressure challenge without NF ECV or ECV (n=5–6 isolated mouse lungs per group, control lungs were ventilated without NF ECV or ECV). Statistical analysis was performed by one-way ANOVA with Tukeys post hoc test. Data are presented as mean±sem.