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. 2023 Jun 22;61(6):2200951. doi: 10.1183/13993003.00951-2022

FIGURE 5.

FIGURE 5

Effect of long-term in vivo exposure to nicotine-containing e-cigarette vapour (ECV) or nicotine-free e-cigarette vapour (NF ECV) on lung function and structure parameters in mice. a–d) Lung function parameters (n=9–11) of static compliance (a), resistance (b), inspiratory capacity (c) and pressure–volume loops (d) of mice exposed to NF ECV, ECV or room air (control) for 8 months. e–j) Lung structure parameters from in vivo micro-computed tomography (CT) measurements (n=9–12) of air/tissue ratio (e), lung density in Hounsfield units (HU) (f) and representative micro-CT imaging (g), or from histological analysis (n=6) of airspace (as a percentage of total area) (h), mean linear intercept (i) and representative pictures (j). Lung sections were stained with haematoxylin and eosin. Scale bars: 250 μm. Statistical analysis: one-way ANOVA with Tukey's post hoc test. Combined p-value from e, f, g, p=0.03 determined by meta-analysis according to Fisher's method using the BisRNA R package. Data are presented as mean±sem.