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. 2023 Jun 8;14:1201129. doi: 10.3389/fpls.2023.1201129

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Copyright © 2023 Uchida, Kim, Sato, Tabeta, Mochida and Hirai

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of isomeric structures of methylhistidine (MH) in seeds of Arabidopsis lines by GC-MS/MS. (A) Chromatographs of τMH and πMH. Trimethylsilyl (TMS) standard samples were analyzed by GC-MS/MS in full scan mode. The total ion chromatograph (TIC) is indicated in blue. (B) Chromatographs obtained by GC-MS/MS in MRM mode. τMH-2TMS (196.00 > 73.10) and πMH-2TMS (218.00 > 73.10) were analyzed in the Col-0 (green), the overexpressing line MT160-OE#32-1 (lime yellow), and extraction buffer (blue). N.D., not detected.