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. 2023 Jun 8;14:1197894. doi: 10.3389/fimmu.2023.1197894

Figure 2.

Figure 2

Platelets from ZIP1/3 DKO mice display increased free [Zn2+]i, but an impaired mobilisation. (A) Determination of intracellular amounts of Zn2+, Ca2+, Mg2+ and Fe2+ by ICP-MS analysis of washed platelets. (B) Representative traces of FluoZin3 labelling of WT and ZIP1/3 DKO platelets and subsequent stimulation with thrombin (0.02 U/ml; indicated by arrow). The respective background signal (F0) was subtracted from each sample to allow for direct comparison of absolute levels and release. (C) Absolute FluoZin3 (MFI) signals after background subtraction, prior to thrombin stimulation (F1-F0). (D) Released (left) and retained (right) intracellular levels of free Zn2+ as determined by FluoZin3 fluorescence upon stimulation of WT or ZIP1/3 DKO platelets with thrombin (0.02 U/ml, 5min). MFI and relative levels, as compared to absolute signals, are depicted. n= 8-10, (Student’s t-test), **P<0.01; *** P<0.001.