Figure 8.

ZIP1/3 deficiency promotes ex vivo and in vivo thrombus formation. Adhesion and thrombus formation of platelets on collagen was assessed in a flow adhesion assay at a wall shear rate of 1000/s. Representative images (A) and the quantification (B) of the surface coverage and the relative thrombus volume are shown (n = 6-7; Student’s t-test; *P<0.05; Scale bar: 50 μm). Thrombus formation upon FeCl₃-mediated injury of mesenteric arteries. Representative images (C) and quantitative analysis (D) indicate the time for appearance of 1^st in vivo thrombus (> 10 µM) and time to occlusion. Each dot represents one mesenteric artery, n=10-13, (Student’s t-test); *P<0.05, **P<0.01.