Skip to main content
. Author manuscript; available in PMC: 2024 Mar 1.
Published in final edited form as: Pharmacol Res. 2023 Feb 10;189:106691. doi: 10.1016/j.phrs.2023.106691

Figure 2. 15-lipoxygenase expression in mast cells is not modulated after cell activation.

Figure 2.

RT-PCR analysis of Alox15 (A) and Alox5 (B) expression in immunoglobulin E-activated (αIgE) and not-activated (NA) laboratory of allergic diseases 2 (LAD-2) cells, preincubated with 20 μM docosahexaenoic acid (DHA), 30 min, 1.5 and 24 h after activation. (*p<0.05, ***p<0.001). (C) Representative histogram of 15-lipoxygenase protein expression in cord blood-derived mast cells (CBMCs) preincubated with 20 μM docosahexaenoic acid (DHA) 30 min after IgE-mediated activation. RT-PCR analysis of Alox15 (D) and Alox5 (E) expression in IgE-activated and not-activated foreskin-derived mast cells (FSMCs), preincubated with 20 μM docosahexaenoic acid (DHA), 1h after activation. Data are expressed as mean ± SEM of three independent experiments.