Inhibitory assessment (IC₅₀ in μM) of the most active inhibitors 4b and 4g on S. aureus DNA gyrase, topoisomerase IV, and dihydrofolate reductase enzymes.

Compound	IC50 (μM)
	S. aureus DNA gyrase	Topoisomerase IV	Dihydrofolate reductase
4c (R = 4-Cl)	2.82 ± 0.13	68.68 ± 1.21	0.23 ± 0.06
4g (R = 4-OCH3)	1.37 ± 0.11	79.15 ± 1.23	0.17 ± 0.03
4h (4-OCH3–3-NO2)	1.25 ± 0.12	67.28 ± 1.21	0.13 ± 0.05
Ciprofloxacin	1.15 ± 0.13	25.42 ± 1.21	—
Methotrexate	—	—	0.36 ± 0.02