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(A) Treg expansion in first year post-transplant (adapted from Posselt, et. al., 2010); (B) Operational tolerance in patient EFA-4 following withdrawal of immunosuppression; (C) EFA4 cytokine production in mixed lymphocyte reaction to donor-specific (black) and third-party (gray) antigen. EFA4 demonstrated no reactivity to donor or third-party antigen in the first 12 months post-transplant, which returned by 24 months.
