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. Author manuscript; available in PMC: 2023 Jun 22.
Published in final edited form as: Cell Rep. 2023 May 8;42(5):112488. doi: 10.1016/j.celrep.2023.112488

Figure 6. Loss of FASN or ACC1, but not ACC2, in Ads reduces de novo FA synthesis in iWAT.

Figure 6.

(A–C) [14C]-glucose conversion into TG FAs was measured ex vivo in iWAT derived from (A) FASN-flox/flox and cAdFASN KO, (B) ACC1-flox/flox and cAdACC1 KO, and (C) ACC2-flox/flox and cAdACC2 KO mice. n = 10 mice per condition. Presented are means ± SEM. Two-tailed Student’s t test was used for statistical analysis between control and KO groups.

(D) The pathways of glucose conversion into glycerol-3-P and palmitate (highlighted) to produce TGs.

(E and F) [14C]-glucose incorporation into TG FAs measured ex vivo in iWAT from (E) FASN-ACC1-flox/flox and cAdFASN-ACC1 DKO and (F) FASN-ACC2-flox/flox and cAdFASN-ACC2 DKO mice. n = 10 mice per condition. Presented are means ± SEM. Two-tailed Student’s t test determined statistical significance.

In (A) and (D), *p < 0.05. See also Figure S5.