KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER | |
|---|---|---|---|
| Antibodies | |||
| Anti-Fatty Acid Synthase, Clone 23 | BD Bioscience | Cat# 610963; RRID:AB_398276 | |
| Anti-Fatty Acid Synthase (C20G5) Rabbit mAb | Cell Signaling Technology | Cat# 3180; RRID:AB_2100796 | |
| Anti-Vinculin (E1E9V) XP | Cell Signaling Technology | Cat# 18799; RRID:AB_2714181 | |
| Anti-GAPDH (D16H11)XP | Cell Signaling Technology | Cat# 8884; RRID:AB_11129865 | |
| Anti-ACC | Cell Signaling Technology | Cat# 3662; RRID:AB_2219400 | |
| Anti-UCP1 | Abcam | Cat# ab10983; RRID:AB_2241462 | |
| Anti-ACLY | Proteintech | Cat# 15421–1-AP; RRID:AB_2223741 | |
| Anti-MLYCD | Proteintech | Cat#15265–1-AP; RRID:AB_2146403 | |
| Anti-Ac-lysine (7F8) | Santa Cruz Biotechnology | Cat# sc-81623; RRID:AB_1118639 | |
| Pan anti-malonyllysine antibody | PTM Biolabs | Cat# PTM-901; RRID: AB_2687947 | |
| Anti-Rabbit IgG antibody, HRP-conjugated | Millipore | Cat# AP307P; RRID:AB_11212848 | |
| Anti-Mouse IgG antibody HRP-conjugated | Millipore | Cat# AP308P; RRID:AB_11215796 | |
| Chemicals, peptides, and recombinant proteins | |||
| Tamoxifen | Sigma-Aldrich | Cat# T5648 | |
| Tween 20 | Sigma Aldrich | Cat# P1379 | |
| Bovine serum albumin (fatty acid free) | Sigma-Aldrich | Cat# A8806 | |
| D-(+)-Glucose | Sigma-Aldrich | Cat# G8270 | |
| Sodium pyruvate | Gibco | Cat# 11360070 | |
| L-Glutamine | Gibco | Cat# 25030081 | |
| [14C]-U-Glucose | Perkin Elmer | Cat# NEC042X250UC | |
| Critical commercial assays | |||
| Enhanced Chemiluminescent Substrate it | Perkin Elmer | Cat# NEL104001EA | |
| iScript™ cDNA Synthesis Kit | Bio-Rad Laboratories | Cat# 1708891 | |
| iTaq Universal SYBR® Green Supermix | Bio-Rad Laboratories | Cat# 1725121 | |
| Deposited data | |||
| RNA-seq - accession number: PRJNA947210 | This paper | https://www.ncbi.nlm.nih.gov/bioproject/PRJNA947210 | |
| Lipidomics datasets, https://doi.org/10.17632/zsvnzhf4hv.2 | This paper | https://data.mendeley.com/datasets/zsvnzhf4hv/2 | |
| Experimental models: Organisms/strains | |||
| Mouse: cAdipoq-cre: B6;FVB-T(IAdipoq-cre)1Evdr/J | The Jackson Laboratory | Strain #010803; RRID: IMSR_JAX:010803 | |
| Mouse: Adipo-Cre: B6.129-Tg(Adipoq-cre/Esr1*)1Evdr/j | The Jackson Laboratory | Strain #024671; RRID: IMSR_JAX:024671 | |
| Mouse: Acl/’ox/flox) | Taconic Bioscience | Model #7917; RRID: IMSR_TAC:7917 | |
| Mouse: Acacafllox/flox) | The Jackson Laboratory | Strain # 030954; RRID: IMSR_JAX:030954 | |
| Mouse: Acacbfllox/flox) | The Jackson Laboratory | Strain #013042; RRID: IMSR_JAX:013042 | |
| Mouse: M/ycdfllox/flox) | Fisher-Wellman et al41 | N/A | |
| Mouse: Fasnfllox/flox) | Guilherme et al.17 | N/A | |
| Mouse: cAdACLYKO | This paper | N/A | |
| Mouse: iAdACLYKO, inducible | This paper | N/A | |
| Mouse: cAdACCIKO | This paper | N/A | |
| Mouse: cAdACC2KO | This paper | N/A | |
| Mouse: cAdMCDKO | This paper | N/A | |
| Mouse: cAdFASNKO | This paper | N/A | |
| Mouse: iAdFASNKO, inducible | Guilherme et al.17 | N/A | |
| Mouse: cAdFASNKO | This paper | N/A | |
| Mouse: cAdFASN-ACLYDKO | This paper | N/A | |
| Mouse: iAdFASN-ACLYDKO, inducible | This paper | N/A | |
| Mouse: cAdFASN-ACC1DKO | This paper | N/A | |
| Mouse: cAdFASN-ACC2DKO | This paper | N/A | |
| Oligonucleotides | |||
| Primers used for Real-time PCR reactions | See Table S1 | https://www.genewiz.com/en | |
| Software and algorithms | |||
| GraphPad Prism 9.5.1 | GraphPad | https://www.graphpad.com/ | |
| Image Lab 5 Analysis Software | Bio-Rad Laboratories | Iten# 1709690 | |
| ImageJ | ImageJ | ImageJ.nih.gov | |
| Adiposoft | Galarraga et al.66 | N/A | |
| CalR | Mina et al.67 | https://calrapp.org/ | |
| ProFAT | Cheng et al.43 | http://profat.genzentrum.lmu.de. | |
| Other | |||
| ChemiDoc™ XRS+ Imaging System | Bio-Rad Laboratories | Model# 1708265 | |
| Leica DM2500 LED optical microscope | Leica Microsystems | Model# DM2500 LED | |
| Leica MC170 HD camera | Leica Microsystems | Model# MC170 HD | |