Fig. 5.

OLT1177 protects dopaminergic neurons from MPTP-mediated cell death. a and b Representative tyrosine hydroxylase (TH) immunostaining in the striatum (St, a) and substantia nigra pars compacta (SNpc, b). Mice were treated with 200 mg/kg of OLT1177 (OLT) for 7 days. Saline was used as vehicle. Positive signal appears in brown color. The area outlined in the box is shown in higher magnification in the inset. c and d TH immunoreactivity in the St (c) and TH stereological counting in the SNpc (d). Scale bar: 500 µm. Data are represented as mean ± SEM. N = 5 for Sham + Saline and N = 7 for both MPTP groups. One-way ANOVA with Tukey’s post hoc correction was used to analyze differences between groups. *p < 0.05, **p < 0.01, and ***p < 0.001