Fig. 2.

DHCR24 knock-in markedly improves cognitive ability of 5xFAD mice. A The timeline of the experiment and fluorescence images of ZsGreen (green) one month after AAV injection (scale bar, 200 μm). B Fluorescence images of Filipin staining (blue) in the CA1 region of hippocampus (scale bar, 10 μm) and the analysis results of mean fluorescence intensity which were normalized with 5xFAD-Control group. C Quantification of cholesterol level in hippocampus two groups by LC–MS/MS analysis which were normalized with 5xFAD-Control group. n = 3 sample/group, every sample was included 2–4 mice randomly. D Escape latency during the 5 days acquisition phase of Morris water maze (MWM) test. E The percentage of time spent in target quadrant in MWM test. F The number of platform crossings in MWM test. G The latency to platform in MWM test. H The swim speed of mice in MWM test. I The number of target quadrant crossings in MWM test. J The time spent in platform in MWM test. K The latency to the target quadrant in MWM test. n = 7–10 mice per group in [G], n = 8–10 mice per group in [D–F and H–K]. Data expressed as mean ± SEM, statistical analysis between the five groups was analyzed by one-way ANOVA with Tukey’s post hoc test, except for escape latency was analyzed by three-way repeated measures ANOVA with LSD post hoc test. *P < 0.05; **P < 0.01; ***P < 0.001; compared with aged-matched 5xFAD-Control group. The images of MWM trials were showed in Additional file 1: Fig. S1