Fig. 3.

The over-expression of DHCR24 in the hippocampus of 5xFAD mice. A, B The fluorescence images of ZsGreen (green) and DHCR24 (red) in hippocampus (scale bar, 10 μm). C Fluorescence image of ZsGreen (green) in hippocampus (scale bar, 200 μm). D The fold change expression of mRNA of DHCR24 in hippocampus by RT-PCR. E The immunoblotting bands of DHCR24 protein and the analysis results of western blot with mean gray value which were quantification on the ratio of target proteins against GAPDH. n = 3 mice per group. F Co-staining of ZsGreen (green) with NeuN (red) and GFAP (red) or Iba1 (red) in hippocampus (scale bar, 10 μm). Data expressed as mean ± SEM, statistical analysis between the two groups was analyzed by unpaired two-tailed Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001; compared with aged-matched 5xFAD-Control group