Fig. 5.

DHCR24 knock-in improves synaptic function in 5xFAD mice. A, B Fluorescence images of Synapsin I in the CA1 and CA3 region of hippocampus (scale bar, 20 μm). C Fluorescence images of Synapsin I in the DG region of hippocampus (scale bar, 50 μm). D–F Mean fluorescence intensity of Synapsin I in the CA1, CA3, and DG region. n = 3 mice/group. G The immunoblotting bands of PSD95, Synapsin I, RhoA, p-MEK and p-ERK in the hippocampus of 5xFAD-Control group and 5xFAD-DHCR24 group. H Analysis of western blot with mean gray value which all were quantification on the ratio of target proteins against GAPDH except p-MEK and p-ERK were the ratio against total MEK and ERK. n = 3 mice/group. Data expressed as mean ± SEM, statistical analysis between the two groups was analyzed by unpaired two-tailed Student’s t-test, between the four groups was analyzed by one-way ANOVA with Tukey’s post hoc test. *P < 0.05; **P < 0.01; ***P < 0.001; compared with aged-matched 5xFAD-Control group. The images of Synapsin I in CA1, CA3 and DG regions of WT-Control group and WT-DHCR24 group were showed in Additional file 2: Fig. S2