Fig. 6.

DHCR24 knock-in inhibits apoptosis or reactive astrocytosis and promotes microglial phagocytosis in 5xFAD mice. A Fluorescence images of TdT-mediated dUTP Nick-End Labeling (TUNEL) staining (red) in the CA1 region of hippocampus (scale bar, 20 μm). B The ratio of TUNEL-positive cells against DAPI. C Representative immunoblotting bands of Cleaved caspase3, Bcl-2, Bim, Bax, p-JNK and p-P38 MAPK and analysis of western blot with mean gray value which all were quantification on the ratio of target proteins against GAPDH except p-JNK and p-P38 MAPK were against total JNK and P38 MAPK. D Co-staining of ZsGreen (green) with GFAP (red) in hippocampus and analyzing with the percentage of fluorescence area of GFAP (scale bar, 20 μm). E Co-staining of Iba1 (green) with CD68 (red) in hippocampus and analyzing with the percentage of CD68 + positive within Iba1 + microglia (scale bar, 10 μm). The arrows indicate CD68 + and Iba1 + microglia. n = 3 mice/group in [A-D], n = 6 brain slices from 3 mice/group in [E]. Data expressed as mean ± SEM, statistical analysis between the two groups was analyzed by unpaired two-tailed Student’s t-test, between the four groups was analyzed by one-way ANOVA with Tukey’s post hoc test. *P < 0.05; **P < 0.01; ***P < 0.001; compared with aged-matched 5xFAD-Control group