Fig. 2.
The [Ca2+]cyt response of Pi‐replete and Pi‐starved intact or cut root tips to control solution. Arabidopsis Col‐0 aequorin‐expressing seedlings were grown on full or zero Pi medium. Root tips (1 cm; 'root tip intact’) or cut root tips (1 cm of root tip with the apical 1 mm cut off) were challenged with control solution applied at 35 s, and [Ca2+]cyt was measured for 155 s. (A) Mechanical stimulation (caused by control solution): time course trace represents mean ± SEM from 2 independent trials, with n = 5 individual intact root tips, and 3 independent trials, with n = 15 individual cut root tips averaged per data point. Time course data were analysed for (B) touch maximum, (C) area under the curve (AUC), all baseline‐subtracted, with each dot representing an individual data point. In the boxplot, each dot represents an individual data point. The thick middle line denotes the median, separating the upper and lower half of the data; the hinges (box outline) denote median of the upper and the lower half of the data, respectively; bars denote entirety of data excluding outliers. anova with post‐hoc Tukey test was used to assess statistical differences. Significance ***P < 0.001, n.s. not significant.