FIG 1.

Generation of ORP1-null HeLa cell line. (A) Schematic representation of ORP1 isoforms ORP1L and ORP1S, showing location targeted by sgRNA used in CRISPR-Cas9 generation of ORP1-null cells. ANK, ankyrin repeat domains; FFAT, two phenylalanines in an acidic tract motif; ORD, ORP-family cholesterol binding domain. (B) ORP1 knockout was confirmed by Western blotting. Absence of ORP1L (108 kDa) and ORP1S (50 kDa) was demonstrated in ORP1-null HeLa cells. GAPDH (35 kDa) was used as a loading control.