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. 2023 Apr 5;8(3):e00019-23. doi: 10.1128/msphere.00019-23

FIG 3.

FIG 3

OPS-IpaB conjugation. (A) Schematic representation of OPS-IpaB conjugation process using IpaB-var1 and DBCO-derivatized OPS. Purified IpaB-var1 was incubated with S. flexneri 2a OPS-DBCO at room temperature (RT), and a click-chemistry reaction was performed to produce OPS-IpaB conjugate. (B) Safe-blue-stained SDS-PAGE analysis showing efficient conjugation of IpaB-var1 to OPS-DBCO. SEC-MALS analysis of the purified conjugates estimates an average molar mass of 285.7 ± 2 kDa for the OPS-IpaB conjugate. var, variant.