Skip to main content
. Author manuscript; available in PMC: 2023 Jun 22.
Published in final edited form as: Bioconjug Chem. 2019 Dec 16;31(1):104–112. doi: 10.1021/acs.bioconjchem.9b00781

Figure 5.

Figure 5.

(A) Cell viability fraction of MDA-MB-468 cells in combination with affibody-enzyme fusion proteins, 5-fluorocytosine prodrug, and UV light, as measured by MTT assay for different affibody-enzyme incubation times. Top dotted line: baseline viability of cells incubated with 5-FC prodrug (0.854). Middle dotted line: baseline viability of cells incubated with 5-FU drug (0.467). Left to right at each timepoint: (+)WT-CodA (+)5-FC (+)UV, (+)N23BP-CodA (+)5-FC (+)UV, (+)CodA (+)5-FC (+)UV, (+)N23BP-CodA (+)5-FC (−)UV, (+)N23BP-CodA (−)5-FC (+)UV, and (+)N23BP-CodA (−)5-FC (−)UV. Error bars represent standard error. (B) Cell viability fraction of MDA-MB-468 cells in combination with affibody-enzyme fusion proteins, 5-fluorocytosine prodrug, and UV light, as measured by MTT assay for different 5-fluorocytosine incubation time points. Left to right at each timepoint: (+) N23BP-CodA, (+) WT-CodA, (+) CodA, (+) 5-FC, (+) 5-FU (no 5-FC). Error bars represent standard error. All experiments were performed in triplicate (n=3).