Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 21;220(9):e20221847. doi: 10.1084/jem.20221847

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Lei et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 7. — Plasticity of T cell states in human breast cancer progression and metastasize to lymph nodes. (A) The UMAP of 14,646 aggregated cancer cells from human primary breast tumors and metLNs. The aggregated cancer cell UMAP is separated into primary tumors (left) and metLNs (right) for visualization. We selected the top 30 PCs for the UMAP analysis with a minimum distance of 0.5 for the display. (B) The expression profiling of MHC-II genes in cancer cells from primary tumors and metLNs. Red represents high expression, and gray represents low expression. The size of the circle represents the proportion of cells expressing the indicated genes in each cluster. (C) The MHC-II gene signature in cancer cells from primary tumors and metLNs. Single-cell MHC-II signature score was measured by UCell. (D) The UMAP of cells from TDLNs and metLNs, colored by cell types. (E) Violin plot shows the anergy and exhaustion gene signature in Tregs in TDLNs and metLNs from all the patients. Gene expression signature score was measured by UCell. The CD4⁺ T cell anergy and exhaustion genes were generated by Trefzer et al. (2021). (F) Violin plots show the gene expression of CD44, MAF, TOX, and TIGIT in Treg cells in the lymph nodes. Wilcoxon rank-sum test was used for statistical analysis; *, P < 0.05; **, P < 0.01; ***, P < 0.001.