Table 2.
Guide-RNAs for CRISPR/Cas9 gene knockout assays
| sgRNA | Original sequence 5′–3′ | BsmBI site added |
|---|---|---|
| Ifngr1 sgRNA 1 | 5′-CCAGGAAAGGTCGGGCCCCC-3′ | 5′-CACCGCCAGGAAAGGTCGGGCCCCC-3′ |
| Complementary | 5′-GGGGGCCCGACCTTTCCTGG-3′ | 5′-AAACGGGGGCCCGACCTTTCCTGGC-3′ |
| Ifngr1 sgRNA 2 | 5′-TGGTATTCCCAGCATACGAC-3′ | 5′-CACCGTGGTATTCCCAGCATACGAC-3′ |
| Complementary | 5′-GTCGTATGCTGGGAATACCA-3′ | 5′-AAACGTCGTATGCTGGGAATACCAC-3′ |
| Ifngr1 sgRNA 3 | 5′-GATCCTACATACGAAACATA-3′ | 5′-CACCGGATCCTACATACGAAACATA-3′ |
| Complementary | 5′-TATGTTTCGTATGTAGGATC-3′ | 5′-AAACTATGTTTCGTATGTAGGATCC-3′ |
| Ifngr2 sgRNA 1 | 5′-TCTGTGATGTCCGTACAGTT-3′ | 5′-CACCGTCTGTGATGTCCGTACAGTT-3′ |
| Complementary | 5′-AACTGTACGGACATCACAGA-3′ | 5′-AAACAACTGTACGGACATCACAGAC-3′ |
| Ifngr2 sgRNA 2 | 5′-TTCAGTATCTTGTCCACTAC-3′ | 5′-CACCGTTCAGTATCTTGTCCACTAC-3′ |
| Complementary | 5′-GTAGTGGACAAGATACTGAA-3′ | 5′-AAACGTAGTGGACAAGATACTGAAC-3′ |
| Ifngr2 sgRNA 3 | 5′-CACTGGTCTTGGGTCATTGC-3′ | 5′-CACCGCACTGGTCTTGGGTCATTGC-3′ |
| Complementary | 5′-GCAATGACCCAAGACCAGTG-3′ | 5′-AAACGCAATGACCCAAGACCAGTGC-3′ |
| Ciita sgRNA 1 | 5′-ATAGAGATCCCTGTAGAAGC-3′ | 5′-CACCGATAGAGATCCCTGTAGAAGC-3′ |
| Complementary | 5′-GCTTCTACAGGGATCTCTAT-3′ | 5′-AAACGCTTCTACAGGGATCTCTATC-3′ |
| Ciita sgRNA 2 | 5′-CCAGTTCAGCAAGCTGTTGC-3′ | 5′-CACCGCCAGTTCAGCAAGCTGTTGC-3′ |
| Complementary | 5′-GCAACAGCTTGCTGAACTGG-3′ | 5′-AAACGCAACAGCTTGCTGAACTGGC-3′ |
| Ciita sgRNA 3 | 5′-TACTGAAGAGGTCCTTGCTC-3′ | 5′-CACCGTACTGAAGAGGTCCTTGCTC-3′ |
| Complementary | 5′-GAGCAAGGACCTCTTCAGTA-3′ | 5′-AAACGAGCAAGGACCTCTTCAGTAC-3′ |
| H2-Aa sgRNA1 | 5′-ATAGCAAGTCAGTCGCAGA-3′ | 5′-CACCGATAGCAAGTCAGTCGCAGA-3′ |
| Complementary | 5′-TCTGCGACTGACTTGCTAT-3′ | 5′-AAACTCTGCGACTGACTTGCTATC-3′ |
| H2-Aa sgRNA2 | 5′-TATACCATAGGTGCCTACG-3′ | 5′-CACCGTATACCATAGGTGCCTACG-3′ |
| Complementary | 5′-CGTAGGCACCTATGGTATA-3′ | 5′-AAACCGTAGGCACCTATGGTATAC-3′ |
| H2-Ab1 sgRNA1 | 5′-CGTATGCGCTGCGTCCCGT-3′ | 5′-CACCGCGTATGCGCTGCGTCCCGT-3′ |
| Complementary | 5′-ACGGGACGCAGCGCATACG-3′ | 5′-AAACACGGGACGCAGCGCATACGC-3′ |
| H2-Ab1 sgRNA2 | 5′-GCGGCCAGACGCCGAGTAC-3′ | 5′-CACCGGCGGCCAGACGCCGAGTAC-3′ |
| Complementary | 5′-GTACTCGGCGTCTGGCCGC-3′ | 5′-AAACGTACTCGGCGTCTGGCCGCC-3′ |
| H2-Eb1 sgRNA1 | 5′-CAACGGGACGCAGCGCGTG-3′ | 5′-CACCGCAACGGGACGCAGCGCGTG-3′ |
| Complementary | 5′-CACGCGCTGCGTCCCGTTG-3′ | 5′-AAACCACGCGCTGCGTCCCGTTGC-3′ |
| H2-Eb1 sgRNA2 | 5′-GACGTGGGCGAGTTCCGCG-3′ | 5′-CACCGGACGTGGGCGAGTTCCGCG-3′ |
| Complementary | 5′-CGCGGAACTCGCCCACGTC-3′ | 5′-AAACCGCGGAACTCGCCCACGTCC-3′ |