Extended Data Fig. 5 |. Single cell transcriptomic analyses of virus-specific CD4+ T cells following PD-L1 blockade.

(a) Serum Viral Titers in mice prior and post 3 anti-PD-L1 treatments. Line graph indicates average +/−SD of four mice per group. Data are representative of 1 single cell experiment with a total of n = 4 mice per group. Unpaired t-test was used to compare isotype pre and post therapy, and between PDL1 pre and post therapy. (b, c) Data are from one scRNA-seq experiment comprised of pooled cells from four individual mice per group. SMARTA cells were FACS-sorted following the third antibody treatment and analyzed by scRNA-seq. (b) Bar graph depicts differential expression of genes in sorted SLAMF1+ TH1 versus CXCR5+ TFH SMARTA cells from bulk RNA-seq at day 7 after LCMV-Armstrong infection. (c) UMAPs show IFNγ and IL10 gene expression.