Table 1.
Comparison of VDJ Gene Usage of TCRs in Biopsies from Patients and in Splenocytes from their Corresponding PDXs
| Patients and PDXs | V Gene |
D Gene |
J Gene |
Malignant Clone (%) |
|---|---|---|---|---|
| TCRBV | TCRBD | TCRBJ | ||
| Patient-1 (MF) | 29-01 | 01-01 | 01-05 | 79.52 |
|
| ||||
| PRS-1-P0 | 29-01 | 01-01 | 01-05 | 99.99 |
|
| ||||
| PRS-1-P1 | 29-01 | 01-01 | 01-05 | 99.99 |
|
| ||||
| PRS-1-P2 | 29-01 | 01-01 | 01-05 | 99.93 |
|
| ||||
| Patient-2 (SS) | 07-02 | unknown | 02-05 | 94.91 |
|
| ||||
| PRS-2-P0 | 07-02 | unknown | 02-05 | 99.85 |
|
| ||||
| PRS-2-P1 | 07-02 | unknown | 02-05 | 99.93 |
|
| ||||
| PRS-2-P2 | 07-02 | unknown | 02-05 | 99.93 |
|
| ||||
| Patient-3 (SS)_clone 1 | 02-01 | 01-01 | 01-04 | 85.99 |
|
| ||||
| PRS-3-P0 | 02-01 | 01-01 | 01-04 | 76.44 |
|
| ||||
| PRS-3-P1 | 02-01 | 01-01 | 01-04 | 74.04 |
|
| ||||
| PRS-3-P2 | 02-01 | 01-01 | 01-04 | 62.41 |
|
| ||||
| Patient-3 (SS)_clone 2 | 04-03 | 01-01 | 01-02 | 0.06 |
|
| ||||
| PRS-3-P0 | 04-03 | 01-01 | 01-02 | 16.35 |
|
| ||||
| PRS-3-P1 | 04-03 | 01-01 | 01-02 | 25.34 |
|
| ||||
| PRS-3-P2 | 04-03 | 01-01 | 01-02 | 37.57 |
Abbreviations: MF, mycosis fungoides; PDX, patient-derived xenograft; SS, Sézary syndrome; VDJ, variable, diversity, joining.
PRS-1, PRS-2, and PRS-3 refer to PDX derived from Patient-1, Patient-2, and Patient-3, respectively. P0 denotes the original mouse receiving cells directly from each patient. P1 and P2 denote the first and second subsequent mice receiving cells passaged from the P0 mice.