Table 1. Experimental design for the longitudinal infection studies (Experiment 1 and 2), where birds from each population were randomly assigned to one of three inoculation treatments.
We collected pre-inoculation samples from all birds, which served as controls. Sample sizes are not even across dose / isolate treatments due to the ethical and logistical limitations of working with wild vertebrates from seven geographically separated populations.
| Years MG Endemic | |||||||
|---|---|---|---|---|---|---|---|
| 20–25 | 10–20 | 0–10 | |||||
| Population | |||||||
| Treatment | AL | IA | VA | CA | WA | AZ | HI |
| Lower-virulence isolate, Low dose | 10 | 10 | 10 | 10 | 10 | 10 | 11 |
| Lower-virulence isolate, High dose | 10 | 10 | 10 | 9 | 10 | 10 | 11 |
| Higher-virulence isolate, Low dose | 10 | 11 | 10 | 10 | 0 | 10 | 10 |