| Genetic reagent (D. melanogaster) |
Canton-S wild type |
Celeste Berg, UW |
N/A |
N/A |
| Genetic reagent (D. melanogaster) |
Mi{Trojan – Gal4}ChAT[MI04508-TG4.0] CG7715[MI04508-TG4.0-X] |
Bloomington 60317 |
RRID:BDSC_60317; FBti0168134 |
ChAT-GAL4 |
| Genetic reagent (D. melanogaster) |
P{pJFRC7-020XUAS-IVS-mCD8::GFP}attP2 |
Bloomington 32194 |
RRID:BDSC_32194; FBti0131936 |
UAS-mcd8::GFP |
| Genetic reagent (D. melanogaster) |
P{w[+mC]=Hml-GAL4.Delta}2, P{w[+mC]=UAS-2xEGFP}AH2 |
Bloomington 30140 |
RRID:BDSC_30140
|
Hml >GFP |
| Genetic reagent (D. melanogaster) |
P{y[+t7.7] w[+mC]=GMR39 A11-GAL4}attP2 |
Bloomington 50034 |
RRID:BDSC_50034
|
39A11-Gal4 |
| Chemical compound |
L-Leucine |
Sigma-Aldrich |
L8000 |
5 mM |
| Chemical compound |
L-Glutamine |
Sigma-Aldrich |
G3126 |
5 mM |
| Chemical compound |
Insulin (Human Recombinant) |
MP Biomedicals |
0219390080 |
0.1 mg/ml |
| Chemical compound |
Alexa Fluor 647 Phalloidin |
ThermoFisher Scientific |
A22287 |
1:50 in PBST |
| Chemical compound |
EdU |
Abcam |
146186 |
2 mg/mL in food |
| Commercial assay or kit |
Click-&-Go Plus EdU 555 Cell Proliferation Assay Kit |
Click Chemistry Tools |
1351 |
N/A |
| Chemical compound |
VECTASHIELD Antifade Mounting Medium |
Vector Laboratories |
H-1000–10 |
|
| Chemical compound |
VECTASHIELD Antifade Mounting Medium with DAPI |
Vector Laboratories |
H-1200–10 |
|
| Software, algorithm |
FIJI |
PMID:22743772
|
RRID:SCR_002285
|
|
