Figure 2.

Changes in Ca²⁺ transients activated by C6-depleted plasma and normal human plasma (NHP) in ARPE-19 cells. (A) Ca²⁺ transients are given as differences to the baseline in fluorescence ratio between the two excitation wavelengths 340 and 380 nm; plasma concentrations were 10%. Data are mean ± SEM. (B–D) Ca²⁺ transient changes at the initial peak phase and the late sustained phase. C6-depleted plasma induced a significantly lower change in intracellular free Ca²⁺ compared to NHP. The horizontal line represents the mean change in Ca²⁺ transients from baseline. ***p < 0.001 (Mann–Whitney U test).