Figure 6.

Changes in Ca²⁺ transients activated by plasma from age-related macular degeneration patients, stratified for Complement factor H (CFH) risk allele status. Plasma from patients CFH 402YH carriers vs. CFH 402HH carriers (2 different patients per risk allele status, 30 cells per patient). All patients were age-matched and had no additional Age-related maculopathy susceptibility 2 (ARMS2) risk allele. Data are mean ± SD. (A) Ca²⁺ transients are given as differences to the baseline in fluorescence ratio between the two excitation wavelengths 340 and 380 nm; plasma was used in concentrations of 10%. (B, C) Ca²⁺ transient changes at the initial peak phase and the late sustained phase. Plasma from CFH 402HH carriers induced a significantly higher change in intracellular free Ca²⁺ in the sustained phase compared to plasma from carriers of CFH 402YH. The horizontal line represents the mean change in Ca²⁺ transients from baseline. *p < 0.05 (Mann–Whitney U test).